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Advanced Course RNA - Course E05
This intensive course explains the various techniques for the isolation, modification, analysis and synthesis of various RNA species from different biological materials and deals in particular with the peculiarities encountered when handling RNA.
Special methods for difficult starting materials are also demonstrated in addition to various techniques for RNA isolation. Optimized techniques for special applications (e.g. northern blots or cDNA synthesis for qPCR and microarray analyses) are also dealt with, along with a wide ranging catalogue of measures for solving problems in everyday laboratory situations.
The course focuses mainly on:
- Isolation techniques for total RNA and mRNA
- Strategies to improve the yield and quality of RNA, even with difficult samples or samples with limited availability
- Techniques for qualitative and quantitative RNA analysis
- Conservation of RNA
- RNA analysis techniques (e.g. Nuclease Protection Assay, Bandshift Assay)
- Optimized cDNA synthesis
- In vitro transcription for the manufacture of synthetic RNA
- RNA interference
This advanced course provides you with sound specialized knowledge and practical experience in the demanding work with RNA, whereby you can ensure the success of your experiments and recognize and correct the causes of frequently occurring problems. The adaptation of your RNA experiments to your subsequent applications is also dealt with in detail, while links are established in particular to the latest techniques, such as RNA interference and in-vitro translation.
This advanced course requires basic knowledge of the structure and properties of nucleic acids.
Duration: 2 days (9:30am - 5:00pm)
Participation fee € 585,-- (plus VAT) incl. work materials, course documentation, confirmation of participation, snacks and beverages.
