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Basic Course Molecular Biology- Course E01
In addition to basic knowledge of biology, this course provides you with an overview of the essential work techniques required in a modern molecular biology lab in order to conduct successful and efficient analyses and experiments.
Starting off with a comprehensive introduction to cell biology, the molecular processes are explained step by step on a cellular level, from genomic DNA through transcription and translation to functional protein. Using this as the basis, the individual molecular biological techniques for obtaining, processing and analyzing nucleic acids and proteins are then discussed intensively, while you can also gain practical experience of the application of these techniques in extensive accompanying experiments
The course programme focuses mainly on:
- Specific reprocessing of various biological samples for molecular biological experiments
- Techniques for nucleic acid isolation (DNA, RNA, mRNA)
- Qualitative and quantitative analysis of nucleic acids
- PCR and PCR-supported techniques (RT-PCR, Multiplex-PCR, qPCR)
- Modification of nucleic acids (e.g. with restriction enzymes)
- Cloning and transformation techniques
- Sequence analysis
- Techniques of gene expression analysis (microarrays, northern blotting)
- Protein isolation
- Protein-based analysis techniques (ELISA, western blotting, 2D-PAGE)
The course programme is rounded off by an introduction to important bioinformatics applications (including database research and primer design) and an overview of other state-of-the-art molecular biological methods in research and diagnostics.
No specific previous knowledge is required for this basic course.
Duration: 3 days (9:30am - 5:00pm)
Participation fee € 825,-- (plus VAT) incl. work materials, course documentation, confirmation of participation, snacks and beverages.
